Funding for cycling will be spent on improving the network of cycle paths across Scotland and providing training for schoolchildren.
Plans for the £6 million funding for cycling allocated in the recently announced 2013-14 Holyrood budget were outlined by transport minister Keith Brown.
The National Cycle Network will be enhanced and local authorities will be given cash to improve cycling infrastructure on smaller roads, he said.
The Land's End to John O'Groats tourist route will be upgraded from Barnton junction in Edinburgh to the Forth Road Bridge, and money will be available for local authorities to improve the cycle route along the A90 between Edinburgh and Fife.
More cycle paths will be created in Glasgow, in time for the Commonwealth Games, and further routes have been identified for improvements in Dundee, Kirkcaldy and Edinburgh.
Mr Brown said: "I want to ensure that people who currently cycle, and those who are considering it, are given even more reason to get about by bike by continuing to improve Scotland's cycling infrastructure, and this money will do that."
The Government would continue to fund schemes such as Bikeability Scotland and Give Me Cycle Space, which teach children about cycling safety, he said.
"We want to ensure all P6/7 pupils have access to on-road cycle training. This additional funding will allow more local authorities to move to on-road training through staff training, recruitment of volunteers and bike library schemes, particularly in more deprived areas."
Ian Aitken, chief executive of the national promotion organisation Cycling Scotland, said: "We are working with local authorities across Scotland to increase the amount of training which is delivered on-road, as opposed to in the playground, as training is far more beneficial when delivered in a live road environment.
"This funding will enable us to support the network of over 4,000 amazing volunteers that are currently delivering cycle training, increase the number of children receiving training on-road and to continue to campaign for greater consideration for cyclists using the road network."